Actin结合蛋白 Spin-Down 检测试剂盒（非肌肉）-Actin Binding Protein Spin-Down Assay Kit (Non-muscle)-细胞分析试剂盒-欧宝体育官方科技有限公司

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中文名称: Actin结合蛋白 Spin-Down 检测试剂盒（非肌肉）

英文名字: Actin Binding Protein Spin-Down Assay Kit (Non-muscle)

供应商: Cytoskeleton

欧宝体育官方货号: BK013

欧宝体育官方报价: ￥询价/KIT30-100assays欧宝体育官方:

欧宝体育官方说明书

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背景资料: 该Actin结合蛋白 Spin-Down 检测试剂盒（非肌肉）欧宝体育官方是Cytoskeleton品牌原装进口，Cytoskeleton品牌是细胞骨架研究专家，专注于生物化学和细胞过程研究中的纯化蛋白和便捷试剂盒开发与生产。欧宝体育官方作为中国区域的总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立专业技术支持团队的高科技生物企业。可以为您提供及时的咨询响应，专业的欧宝体育官方和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。欧宝体育官方致力于为您提供有价值的欧宝体育官方和服务，在意您的成功！

欧宝体育官方描述: 收到该Actin结合蛋白 Spin-Down 检测试剂盒（非肌肉）Actin Binding Protein Spin-Down Assay Kit (Non-muscle)欧宝体育官方后，查看厂家提供的欧宝体育官方说明书详细介绍。

欧宝体育官方特点: The actin binding protein spin-down assay kit provides G- or F-actin plus positive (α-actinin ) and negative (Bovine Serum Albumin, BSA) binding control proteins. Actin binding occurs when there is an affinity for any site of actin. F-actin binding can be measured by using a spin down method. In this way centrifugation is used to separate F-actin from G-actin by differential sedimentation. F-actin binding proteins will co-sediment with actin filaments and form a pellet at the bottom of the centrifugation tube. Proteins with F-actin bundling activity can be detected since they will cause the F-actin to sediment at lower centrifugal forces than normal (14,000 x g vs 150,000 x g). F-actin severing proteins, G-actin binding proteins or non-actin binding proteins will stay in the supernatant. Severing proteins will be expected if more G-actin remains in the supernatant than in the negative control sample, and this activity should be tested further by measuring F-actin length distributions before and after adding the test protein. G-actin binding proteins can be measured by adding the test protein to G-actin and inducing polymerization, if the test protein sequesters G-actin then during the spin-down assay more actin will be left in the supernatant compared with the control. Actin can exist in two forms: Globular subunit (G-actin) and Filamentous polymer (F-actin) (See the About Actin page for more information). Both forms of actin interact with a plethora of proteins in the cell. To date there are over 50 distinct classes of Actin-Binding Proteins (ABPs), and the inventory is still far from complete. Actin Binding Proteins allow the actin cytoskeleton to respond rapidly to cellular and extracellular signals and are integral to cytoskeletal involvement in many cellular processes. These include cell shape and motility, muscle contraction, intracellular trafficking, cell pathogenesis and signal transduction. This kit contains non-muscle actin (Cat. # APHL99). The same kit is also available with skeletal muscle actin (Cat. # AKL99), see Cat. # BK001. The skeletal muscle actin spin-down kit may be more appropriate to use to study actin binding proteins from muscle tissues.

保存建议: 厂家推荐常温运输，收到欧宝体育官方后建议保存于4°C。

其他: Cytoskeleton公司成立于1993年，专注于生物化学和细胞过程研究中的纯化蛋白和便捷试剂盒开发与生产。公司提供药物筛选、信号转导、蛋白质转录后修饰（PTM）、细胞骨架研究相关的系列试剂盒和欧宝体育官方，尤其以细胞骨架相关研究见长，既能满足于样品较少的科学研究，也可以用于小规模筛选研究和高通量大规模筛选研究。此外，公司还提供微管蛋白，肌动蛋白，小G蛋白，GAPs，GEFs等现有欧宝体育官方的药物筛选服务。