Sar1 belongs to a small GTPase superfamily and GTP binging activity. This protein is involved in intracellular protein transport. There are two different non-clathrin-coated vesicles that are responsible for transport between ER and the Golgi. Coat protein complexes involving membrane-associated GTP binging proteins - Arf1 and Sar1p for COPI and COPII are needed for formation of COP-coated vesicles. Sar1p is a cytosolic protein. It is temporarily recruited onto the membranes of the ER.
应用类型
Immunogold (IG), Western blot (WB)
免疫原
GST fusion of full length recombinant Sar1 of Arabidopsis thaliana UniProt: Q8VYP7, TAIR: At3g62560