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worthington 垂体组织分离指南

发布者：欧宝体育官方科技发布时间：2022-06-09

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原代细胞分离中，针对垂体组织涉及到垂体，滤泡，生长激素、垂体前叶和结节等等的解离通常会使用酶，如何选择合适的酶来完成对应垂体组织的解离，以及原代细胞采集的常规操作， 欧宝体育官方针对垂体相关的组织分类解离分种属、不同的细胞、酶组合推荐，推荐浓度、培养基以及参考文献，详细汇总指南：垂体组织分离指南.png

本的技术手册详情了基准进行化学检测步骤；为创立受损细胞破乳措施供给了一大种因人而异思维逻辑的进行检测工艺；分句出了诸多企业特异形参看数据，以让联合开发有郊的工艺。更加多别的企业的解离手册，点击率观察：各组织分离指南。

垂体
种属	种属详情	细胞名称	酶组合推荐，含推荐浓度	培养基	参考文献
牛	Calf, male, less then 6 months old	垂体 / Pituitary	Collagenase: 0.3%	DMEM	Hassan, H., and Merkel, R.: Perifusion Model System to Culture Bovine Hypothalamic Slices In Series with Dispersed Anterior Pituitary Cells, In Vitro Cell Dev Biol 30A, 435, 1994
牛	Bovine	垂体 / Pituitary	Collagenase: 0.1%	EBSS, CMF	Mason, W. and Ingram, C.: Techniques for Studying the Role of Electrical Activity in Control of Secretion by Normal Anterior Pituitary Cells, Vol. 124,, , 207, 1986
牛	Bovine	滤泡，垂体前叶和结节 / Follicular, anterior pituitary and pars tuberalis	Deoxyribonuclease I: 200 /ml	HBSS, CMF	Ferrara, N., Goldsmith, P., Fujii, D., and Weiner, R.: Culture and Characterization of Follicular Cells of the Bovine Anterior Pituitary and Pars Tuberalis, Vol. 124,, , 245, 1986
牛	Calf, male, 1-6 week old	垂体 / Pitituary	Hyaluronidase: 0.1%	DMEM	Ridgway, E., Klibanski, A., Marorana, M., Milbury, P., Kieffer, J., and Chin, W.: The Effect of Somatostatin on the Release of Thyrotropin and its Subunits from Bovine Anterior Pitituary Cells in Vitro, Endocrinology 112 (6), 1937, 1983
小鼠	Mouse	垂体 / Pituitary	Collagenase Type 2: 0.5%； Deoxyribonuclease I: 0.005%	HBSS	Perez Millain, M., Brinkmeier, M., Mortensen, A. and Camper, S.: PROP1 Triggers Epithelial-Mesenchymal Transition-Like Process in Pituitary Stem Cells., Elife 5, , 2016
小鼠	Mouse	垂体 / Pituitary	Collagenase Type 1: 1,000 u/ml； Soybean Trypsin Inhibitor: 0.01%； Hyaluronidase: 0.1%； Deoxyribonuclease I: 0.001%	DMEM	Pyczek, J., Buslei, R., Schult, D., Holsken, A., Buchfelder, M., Hess, I., Hahn, H. and Uhmann, A.: Hedgehog Signaling Activation Induces Stem Cell Proliferation and Hormone Release in the Adult Pituitary Gland., Sci Rep 6, 24928, 2016
小鼠	Mouse, male	垂体 / Pituitary	Collagenase: 0.4%； Hyaluronidase: 0.1%； Trypsin: 0.3%	DMEM/Han's F12	Steveson Tami C, Ciccotosto Giuseppe D, Ma Xin-Ming, Mueller Gregory P, Mains Richard E, Eipper Betty A: Menkes protein contributes to the function of peptidylglycine alpha-amidating monooxygenase, Endocrinology 144, 188-200, 2003
绵羊	Ovine, adult	生长激素 / Somatotropes	Collagenase Type 1: 0.3%； Hyaluronidase:	Medium 199	Xu Ruwei, Wang Qinling, Yan Ming, Hernandez Maria, Gong Changhong, Boon WahChin, Murata Yoko, Ueta Yoichi, Chen Chen: Orexin-A augments voltage-gated Ca2+ currents and synergistically increases growth hormone (GH) secretion with GH-releasing hormone in primary cultured ovine somatotropes, Endocrinology 143, 4609-19, 2002
大鼠	Rat, male, 12-15 week	垂体 / Pituitary	Collagenase Type 2: 0.4%； Deoxyribonuclease I: 0.04%	DMEM	Akieda-Asai, S., Zaima, N., Ikegami, K., Kahyo, T, Yao, I., Hatanaka, T., Iemura, S., Sugiyama, R., Yokozeki, T., Eishi, Y., Koike, M., Ikeda, K., Chiba, T., Yamaza, H., Shimokawa, I., Song, S., Matsuno, A., Mizutani, A., Sawabe, M. Chao, N., Tanaka, M., : SIRT1 Regulates Thyroid-Stimulating Hormone Release by Enhancing PIP5K gamma Activity through Deacetylation of Specific Lysine Residues in Mammals., PLoS ONE 5, e11755, 2010
大鼠	Rat, Wistar, male, 2 month old	垂体 / Pituitary	Trypsin: 0.1%	DMEM	Zhou, X., De Schepper, J., De Craemer, D., Delhase, M., Gys, G., Smitz, J., and Hooghe-Peters, E.: Pituitary Growth Hormone Release and Gene Expression in Cafeteria-Diet-Induced Obese Rats, J Endocrinol 159, 165, 1998
大鼠	Rat, SD, female, 200 - 250 g	垂体前叶 / Anterior pituitary gland	Trypsin: 0.1%	EBSS, CMF	D'Emden, M. and Wark, J.: Culture Requirements for Optimal Expression of 1,25-Dihydroxyvitamin D3-Enhanced Thyrotropin Secretion, In Vitro Cell Dev Biol 27, 197, 1991
大鼠	Rat	垂体 / Pituitary	Trypsin: 0.3 %	HEPES	Wilfinger, W., Larsen, W., Downs, T., and Wilbur, D.: An In Vitro Model for Studies of Intercellular Communication in Cultured Rat Anterior Pituitary Cells, Tissue Cell 16 (4), 483, 1984
大鼠	Rat, SD, male, 250-450 g	垂体前叶 / Anterior pituitary	Trypsin: 0.25%	Krebs	Portanova, R., Smith, D., and Sayers, G.: A Trypsin for the Preparation of Isolated Rat Anterior Pituitary, Proc Soc Exp Biol Med 133, 573, 1970