尿液外泌体纯化和RNA大提试剂盒（提取试剂盒）| Urine Exosome Purification and RNA Isolation Maxi Kit-细胞分析试剂盒-欧宝体育官方科技有限公司

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欧宝体育官方描述: Norgen’s Exosome Purification and RNA Isolation Maxi Kit constitutes an all-in-one system for the purification of exosomes and the subsequent isolation of exosomal RNA from different urine sample volumes ranging from 11 mL to 30 mL. The purification is based on spin column chromatography that employs Norgen’s proprietary resin. The kit is designed to isolate all sizes of extracellular vesicle RNA, including microRNA. The kit provides a clear advantage over other available kits in that it does not require any special instrumentation, protein precipitation reagents, extension tubes, phenol/chloroform or protease treatments. Moreover, the kit allows the user to elute into a flexible elution volume ranging from 50 µL to 100 µL. The purified RNA is free from any protein-bound circulating RNA and of the highest integrity. The purified RNA can be used in a number of downstream applications including real time PCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays.

欧宝体育官方特点: Purification and enrichment of intact urinary exosomes for functional studies; Bind and elute all RNA irrespective of size or GC content, without bias; Isolate all sizes of exosomal and extracellular vesicle RNA, including microRNA; Versatile urine input volume (11 mL to 30 mL); No phenol extractions, Proteinase K treatment, nor carrier RNA required; No time-consuming ultracentrifugation, filtration nor special syringes are required; No precipitation reagents nor overnight incubation required; Compatible with urine from any species; Pure exosomes are purified and are free-from any other RNA-binding proteins.

其他: Norgen Biotek成立于1998年，公司总部位于加拿大，是一家著名全球化生物技术公司，基于专利的碳化硅膜纯化技术和无苯酚专利配方，生产高品质的核酸和蛋白质纯化及富集欧宝体育官方用于研究和诊断。Norgen Biotek 在2003年获得加拿大国家研究委员会颁发的科技创新奖，是一家致力于样品制备并获得ISO 9001（欧宝体育官方质量）、ISO 13485（欧宝体育官方可用于商业化的医疗设备，包括用于体外诊断领域）和ISO 15189（可用于临床检测机分子诊断领域的研发能力）认证的生物科技公司。目前该公司的欧宝体育官方多达700余种，提供一流的样品制备试剂盒，应用于基因组和蛋白组等不同的领域。其欧宝体育官方主要覆盖包括各种标本类型的DNA，RNA和蛋白质的纯化富集，从活检样本（包括尿液，血浆，血清和血液）中纯化RNA和DNA，全系列的PCR试剂、缓冲液、分子量ladder和塑料过滤装置。